Mass spectrometric imaging (MSI) provides two-dimensional mass spectrometric data enabling to visualize the distribution of analytes e.g. on tissue slices.
We have established MSI on a MALDI-TOF instrument. Here, the tissue is placed on a conductive slide and coated with a suitable MALDI matrix prior to the MS measurement.
Typical analytes for MALDI-MSI are:
- Peptides (tryptic and non-tryptic)
- Small proteins (max. up to 30 kDa)
- Glycans and glycopeptides
- Lipids
- other metabolites
Please note that due to the lack of separation prior to the MS measurement, analytes need to be high abundant and/or easy to ionize to be measured in a MALDI-MSI experiment.
MALDI-MSI measurements can be performed in research cooperation projects. If you are interested in this technique, please write an email to Maria Riedner.